2 edition of new method for inoculation of lettuce with Bremia lactucae found in the catalog.
new method for inoculation of lettuce with Bremia lactucae
R. G. Kapooria
|Statement||by R. G. Kapooria and F. Tjallingii.|
|Series||Instituut voor Plantenziektenkundig Onderzoek. Mededeling no. 514|
|Contributions||Tjallingii, Frans, joint author.|
|LC Classifications||SB599 .W27 no. 514|
|The Physical Object|
|Number of Pages||226|
|LC Control Number||76593555|
Oösporen vanBremia lactucae kunnen worden verkregen door bladschijfjes van sla te inoculeren met een gemengde conidiënsuspensie van twee fysio's van de schimmel, die de twee compatibiliteitstypen (B1 en B2) vertegenwoordigen. Wanneer het bladweefsel geheel vergaan is, worden de schijfjes met oösporen samen met het onderliggende filtreerpapier op een dunne laag grond in een Cited by: Lettuce downy mildew caused by Bremia lactucae is the most important disease of lettuce globally. This oomycete is highly variable and rapidly overcomes resistance genes and fungicides. The use of Cited by: 7.
After incubation, ml portion of enrichment broth was used to DNA extraction for detection of Shigella as previously described. Lettuce inoculation experiment (culture, direct-PCR and enriched-PCR) was performed by triplicate in each dilution (10 to 10 7 CFU/ml) and with 10 repetitions for dilutions 10 3, 10 4 and 10 5 CFU/ml (Enriched-PCR. The occurrence of five distinct physiologic races of Bremia lactucae, the agent of downy mildew of lettuce, is stated to be definitely established by observations and experiments in California [R.A.M., xii, p. ], while other evidence points to the existence of six or seven [ibid., xviii, p. 8]. In this paper the inheritance of immunity from race 5 among the progeny (down to F3) of crosses Cited by:
Enter your email address below. If your address has been previously registered, you will receive an email with instructions on how to reset your password. If you don't receive an email, you should register as a new Cited by: 4. Introduction. Lettuce (Lactuca sativa L.) downy mildew, caused by the oomycete Bremia lactucae Regel, is a major threat to lettuce production around the world [1–2].The life cycle of ae, an obligate biotrophic parasite, involves primary (sexual) and secondary (asexual) infection sexual cycle results in soilborne oospores that are the likely source of primary inoculum .Cited by: 3.
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Voor het uitvoeren van proeven metB. lactucae en voor het toetsen van slarassen en- lijnen bij de resistentieveredeling tegen valse meeldauw is dit een belangrijke verbetering. A new method for inoculation of lettuce with Bremia lactucae | SpringerLinkCited by: 1.
A new method for inoculation of lettuce with Bremia lactucae: Author(s) Kapooria, R.G.; Tjallingii, F. Source: Wageningen: [s.n.] (Mededeling / Instituut voor plantenziektenkundig onderzoek no. ) - 3: Department(s) Research Institute for Plant Protection: Publication type: Research report: Publication year: Keyword(s) lactuca sativa Cited by: 1.
A new method for inoculation of lettuce with Bremia lactucae By R.G. Kapooria and F. Tjallingii Topics: lactuca sativa, slasoorten, lettucesAuthor: R.G.
Kapooria and F. Tjallingii. Method Optimization Studies on Inoculation and Isolation of the Causal Agent of Lettuce Downy Mildew (Bremia Lactucae) Article (PDF Available) January.
Failure to control Bremia lactucae (lettuce downy mildew) with metalaxyl in an intensive lettuce-producing region of Lancashire at the end of was shown to be due to the occurrence of a high. lettuce downy mildew (Bremia lactucae), by means of a new computer package: Jo inMap.
Plant J Cluster analysis was based on Jaccard’s distance coefficients using the method of unweighted. Bremia lactucae is a plant pathogen.
This microorganism causes a disease of lettuce (Lactuca sativa) denominated as downy other strains can be found on 36 genera of Asteraceae including Senecio and ments using sporangia from hosts do not infect lettuce and it is concluded that the fungus exists as a quantity of host-specific strains (formae speciales).Class: Oomycetes.
The objective of this paper was to report new races of lettuce downy mildew causal agent Bremia lactucae in São Paulo State. Downy mildewof lettuce: further L. sativa will be overcome by changes in the viru-investigation of strains of Bremia lactucae occurring lence characteristics of the pathogen population in by: During recent years, the progressive variability of Bremia lactucae pathogens has increasingly challenged the breeding of new downy mildew-resistant lettuce cultivars.
However, conventional plant breeding is a complex and long-lasting process. Especially resistance-testing of newly obtained lettuce lines is still based on visual rating procedures and, hence, labour-intensive and by: Download PDF: Sorry, we are unable to provide the full text but you may find it at the following location(s): (external link) ; http Author: R.G.
Kapooria and F. Tjallingii. Heavy infection of lettuce seedlings by Bremia lactucae spores was induced by 24 hr. incubation (minimum period may be as low as 4 hr.) in a saturated atmosphere; this was followed by 7–10 days under ordinary conditions (i.e. humidity not controlled), and finally by 1–2 days' exposure to high humidity to encourage sporulation.
Infection following different temperature treatments during Cited by: Oospores of Bremia lactucae were capable of germination in vitro from 23 days to 12 months after formation in lettuce cotyledons inoculated with a mixture of conidia of isolates of compatible mating-type.
Rotted lettuce cotyledons containing oospores were stored in tap water at 24 °C. Periodically, over the course of a year, viable oospores were teased from tissue by micromanipulation and Cited by: 8. The formation of oospores by Bremia lactucae Regel in detached cotyledons and seedlings of Lactuca sativa L.
(lettuce) cv. Trocadero Improved is described. Antheridia were attached perigynously to oogonia, and meiotic division of the nuclei in both structures occurred during their by: Bremia lactucae transcriptome sequencing. Two sources of RNA were used for B.
lactucae transcriptome sequencing: RNA isolated from asexual B. lactucae conidiospores and RNA isolated from B. lactucae‐infected lettuce effector genes are known to be differentially expressed during different stages of infection, with transcripts of early‐stage effectors being present in spores Cited by: The oomycete Bremia lactucae also caused a considerable patchy decrease of F V /F M in infected lettuce leaf discs (Bauriegel et al., ), as well as an increase in NPQ and a reduction in both.
Lettuce downy mildew caused by Bremia lactucae is the most important disease of lettuce worldwide. Breeding for resistance to this disease is a major priority for most lettuce breeding programs. Many genes and factors for resistance to B. lactucae have been reported by multiple researchers over the past ~50 years.
Their nomenclature has not been coordinated, resulting in Cited by: Abstract. A total of genotypes of wild Lactuca spp. serriola, L. saligna, and L. aculeata), originating from Israel, Jordan, East Turkey, Armenia, Kazakhstan, China, and various other countries in Europe were screened for resistance to the lettuce downy mildew (Bremia lactucae Regel).
The genotypes of L. serriola (N = ); L. saligna (N = 43); and L. aculeata (N = 22) were tested at Cited by: General information about Bremia lactucae (BREMLA) Name Language; downy mildew of lettuce: English: Falscher Mehltau: Salat: German. BABA induced local and systemic resistance in lettuce (Lactuca sativa) against the Oomycete Bremia lactucae.
Structure-activity analysis showed no induced resistance by related amino-butanoic acids or β-alanine. The R-enantiomer of BABA induced resistance whereas the S-enantiomer did not, suggesting binding to a specific receptor. Other compounds known to be involved in SAR signaling Cited by:. The invention relates to a lettuce plant (Lactuca sativa L.) comprising a resistance allele which confers a broad spectrum resistance to Bremia lactucae, wherein the resistance allele is located on linkage group 9, and which resistance allele is as found in the genome of plants grown from seeds of which a representative sample was deposited under accession number NCIMB Author: Marie Jeanette Willemijn Jeuken, Erik Den Boer, Richard Gerardus Franciscus Visser, Rients Engelhard.Preparation of Bremia lactucae‐infected lettuce leaves for transcriptome analysis.(A) Time line of sampling.
Light grey bars represent periods in which infected lettuce was kept at high humidity. (B) Spores were spray inoculated at time point 0 and at a second time point at 3 days post‐inoculation (dpi).Cited by: The invention relates to a lettuce plant (Lactuca sativa L.) which may comprise a resistance allele which confers a broad spectrum resistance to Bremia lactucae, wherein the resistance allele is located on linkage group 9, and which resistance allele is as found in the genome of plants grown from seeds of which a representative sample was deposited under accession number NCIMB Author: Marie Jeanette Willemijn Jeuken, Erik Den Boer, Richard Gerardus Franciscus Visser, Rients Engelhard.